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Dna Replication

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DNA Replication, Translation, Transcription, and Death Cap Mushrooms
Biochemistry – GRT1
Task 1

(Wolfe, DNA Replication, 2000)

(Wolfe, DNA Translation, 2000)

A death cap mushroom, also known as Amanita phalloides, is a poisonous mushroom that can be detrimental to our health. When a death cap mushroom is ingested a toxic substance known as alpha amanitin is released into the body’s system. This toxin prevents protein production and leads to cell death (Santi, Luca, Caterina Maggioli, Marianna Mastroroberto, Manuel Tufoni, Lucia Napoli, and Paolo Caraceni, 2012). RNA polymerase, the enzyme responsible for synthesizing mRNA, is the enzyme directly affected by alpha amanitin. Alpha amanitin blocks RNA polymerase from working properly within the cell (Hudon-Miller, 2013). Without RNA polymerase, mRNA is not transcribed from DNA. Without mRNA, ribosomes cannot complete translation to form polypeptides, also known as proteins (Hudson-Miller, 2013). Proteins provide the necessary means to keep cells functioning accordingly. The liver and kidneys are the organs targeted by this toxin as they are the filtering system of the body and this toxin will enter their cells (Hudson-Miller, 2013). With RNA polymerase inhibited, no ability to produce proteins, the ultimate effect of this toxin has been reached and cell death occurs.


Hudon-MIller, S. (2013). Death cap mushroom. Retrieved from
Santi, Luca, Caterina Maggioli, Marianna Mastroroberto, Manuel Tufoni, Lucia Napoli, and Paolo Caraceni. "Acute Liver Failure Caused by Amanita phalloides Poisoning." International Journal of Hepatology. 2012.Article ID 487480 (2012): 6 pages. Web. 14 Feb. 2015. <>.
Wolfe, G. (2000). Thinkwell Biochemistry. DNA…...

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